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簡要描述:Chromotek/Cell Cycle Chromobody®plasmid New way to trace cell cycle progression in live cells non-invasively
Chromotek/Cell Cycle Chromobody®plasmid
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Thermofisher旗下Gibco培養(yǎng)系列、ABI細胞計數(shù)儀及PCR儀、Invitrogen分子生物學試劑及Qubit3.0、Ambion的RNA相關(guān)試劑、Molecular probes試劑、Pierce蛋白相關(guān)試劑盒、Nanodrop One/OneC;Parafilm封口膜;Viskase透析袋;Sigma-Aldrich試劑;AccuStandard 標準品;Millipore抗體;Glen Research 核酸合成試劑;Bio-Rad電泳儀及PCR儀;Nanocs的PEG聚合物; Chromotek羊駝抗體; Polysciences轉(zhuǎn)染試劑; Echelon-inc 脂類研究試劑盒; R&D Systems抗體及Elisa試劑盒; Jackson二抗及封閉血清; Biotium 熒光染料;NEB內(nèi)切酶及NGS相關(guān)產(chǎn)品; Athens Research & Technology人類蛋白及抗血清; Illumina二代測試試劑盒; Tocris 小分子試劑; TwistDx恒溫擴增試劑盒(RPA); Enzymatics二代測序酶;Novus抗體及Elisa試劑盒;AG scientific潮霉素B、G-418;Plantmedia植物培養(yǎng)系列及抗體;Agilent 2100生物分析儀;Biolog耗材;Biomatik的Elisa試劑盒;Covaris基因打斷儀;CST抗體;Dharmacon的siRNA產(chǎn)品。
Chromotek/Cell Cycle Chromobody®plasmid
Chromotek/Cell Cycle Chromobody®plasmid
New way to trace cell cycle progression in live cells non-invasively
With the CCC plasmid you obtain the sequence of the Alpaca antibody to PCNA fused to TagRFP (Evrogen). Upon transformation, your cells express the CCC Chromobody® and PCNA is visualized by binding of the fluorescent Chromobody®. The transient binding does not influence PCNA function and thus offers you the unique possibility to observe cell cycle progression in your cells.
Use the CCC to:
Perform dynamic, non-invasive, multi-parameter assays
Multiplex with other procedures, including antibody stainings
Analyze the effect of compounds
transfect
cell line
With the Cell Cycle Chromobody® (CCC) plasmid you can observe cellular processes in response to external stimuli in your cell line of interest.
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