AAT Bioquest公司熒光探針Fluo-8®使用說(shuō)明

 蘇州螞蟻淘生物科技有限公司是AATBIO的中國(guó)大陸區(qū)授權(quán)代理商

產(chǎn)品介紹：       

         染料DiI, DiO, DiD 和 DiR是一類親脂性熒光染料家族，用于標(biāo)記細(xì)胞膜和疏水性組織。這是一類環(huán)境敏感型熒光染料，當(dāng)它們與膜結(jié)合或者與親脂性生物分子(例如蛋白質(zhì)，雖然在水中其熒光強(qiáng)度很弱)結(jié)合時(shí)，其熒光強(qiáng)度顯著增強(qiáng)。它們具有很高的淬滅系數(shù)，偏光依賴性和很短的激發(fā)壽命。一旦應(yīng)用于細(xì)胞中，這種染料會(huì)在細(xì)胞內(nèi)質(zhì)膜中逐步擴(kuò)散，導(dǎo)致在其*濃度條件下，將整個(gè)細(xì)胞染色。

        它們不同的熒光顏色：DiI（橙色熒光）、DiO（綠色熒光）、DiD（紅色熒光）、DiR（深紅色熒光），為活細(xì)胞多色彩熒光成像分析和流式細(xì)胞術(shù)提供了一種便捷的工具。DiO和DiI可以分別與標(biāo)準(zhǔn)的FITC和TRITC濾光器一同使用，其中，DiO可以被633 nm He-Ne激光激發(fā)，并且具有比DiI更長(zhǎng)的激發(fā)和發(fā)射光波長(zhǎng)，為標(biāo)記細(xì)胞和組織的那些本身就具有本底熒光的染料提供了非常的替代品。

        DiR在活體成像或者示蹤中非常有用，因?yàn)樗鼈兯l(fā)射的紅外光可以高效地穿過(guò)細(xì)胞和組織，并且在紅外光范圍內(nèi)，其本底熒光水平很低。

技術(shù)資料：

      1.Ex(nm)：748

      2.Em(nm)：780

      3.分子量：

      4.溶劑：DMSO

AAT Bioquest公司熒光探針Fluo-8®使用說(shuō)明

 Fluo-8® AM is a novel green fluorescent indicator that utilizes a fluorescein core to monitor Ca2+ concentration and flux in cells. It has several key advantages over earlier generations of indicators like Fluo-3 AM and Fluo-4 AM:

• Enhanced Intensity → 2x brighter than Fluo-4 AM
• Better & Faster Loading → Room temperature instead of 37°C
• More Robust Results → Probe effective in a wide range of temperatures
• Variety of K_d → Many options for calcium binding dissociation constant

Fluo-8® AM Features

Fluo-8® AM is a novel fluorescent green calcium indicator that builds upon the fluorescein core while rectifying the issues that plagued earlier indicators like Fluo-3 AM and Fluo-4 AM. In particular, it has several key advantages:

• Enhanced Intensity - Tests have shown Fluo-8® AM is twice as bright as Fluo-4 AM and up to four times brighter than Fluo-3 AM
• Better Loading - Fluo-8® AM can be loaded at room temperature, unlike the 37°C incubation required by Fluo-4 AM
• Faster Loading - Room temperature loading means no need to warm up and cool down cells; finish loading in as little as 20 minutes
• Robust - Fluo-8® is less temperature dependent than other probes, leading to more consistent results
• Versatile K_d - Fluo-8® AM is available with a variety of Ca2+ binding dissociation constants, see Table 1 for details

The most brilliant achievement of Fluo-8® AM is that it improves upon the shortcomings of Fluo-3 AM and Fluo-4 AM without touching the excitation and emission. Like Fluo-3 AM and Fluo-4 AM, Fluo-8® AM will excite at ~490 nm and emit at ~520 nm. This allows for a seamless transition for those who are familiar with Fluo-3 AM and Fluo-4 AM but wish to upgrade to Fluo-8® AM. 

As with its predecessors, Fluo-8® AM is completely compatible with use in flow cytometry, fluorescence microscopy, fluorescence spectroscopy and the fluorescence microplate platform. To read more about how Fluo-8® AM has revolutionized calcium imaging, or to see additional Fluo-8® product applications, please click here. 

Table 1. Ca2+ Binding Dissociation Constants