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cellscript公司全系列產(chǎn)品

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  • 發(fā)布日期：2024/6/27
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cellscript公司全系列產(chǎn)品

 

 Cellscript™的使命是為臨床研究和治療提供*的產(chǎn)品和技術(shù)，用于制造和使用RNA在細胞中進行翻譯。

    目前的產(chǎn)品包括用于體外轉(zhuǎn)錄的試劑盒、使用cap模擬物或cap酶的5' RNA capping和3' RNA聚腺苷酸化的試劑盒，以及用于制造用于細胞翻譯的cap、poly(a)-tail mRNA的all-in-one試劑盒。

    Cellscript™近推出了隱身™的體外合成RNA的RNA轉(zhuǎn)錄套件包含修飾核苷,如假尿苷(Ψ)和/或5-methylcytidine (m5C)代替相應(yīng)的U或C規(guī)范核苷(圖1)。這些工具都取名為“因為封頂,聚腺苷酸和核苷修飾的RNA產(chǎn)物(稱為“INCOGNITO mRNAs”)是經(jīng)過偽裝的，因此當(dāng)它們被轉(zhuǎn)染到表達多種RNA傳感器的哺乳動物細胞中時，不會像相應(yīng)的未經(jīng)修飾的mrna一樣，引發(fā)先天免疫反應(yīng)。

產(chǎn)品清單：

 

cellscript公司全系列產(chǎn)品

 

mRNA Production


T7 mScript™ Standard mRNA Production System  (PDF)

Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page.
C-MSC1161010 reactions$320
C-MSC10062525 reactions$702

 


In Vitro Transcription


T7-FlashScribe™ Transcription Kit  (PDF)

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in
30 minutes.
C-ASF350750 reactions$286


T7-Scribe™ Standard RNA IVT Kit  (PDF)

For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.
C-AS310750 reactions$267


SP6-Scribe™ Standard RNA IVT Kit  (PDF)

For the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours.
C-AS310650 reactions$267


Post-Transcriptional Capping


ScriptCap™ m7G Capping System  (PDF)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching *. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce
Cap 1 caps, that which is found on most eukaryotic mRNAs.
C-SCCE062525 reactions$436


ScriptCap™ 2'-O-Methyltransferase Kit  (PDF)

For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m7G Capping System to produce virtually * Cap 1-capped RNA in a single reaction.
C-SCMT062525 reactions$219


ScriptCap™ Cap 1 Capping System  (PDF)

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching *. This cannot be accomplished with co-transcriptional capping methods.
C-SCCS171010 reactions$211


Co-Transcriptional Capping


MessageMAX™ T7 ARCA-Capped Message Transcription Kit  (PDF)

For co-transcriptional capping of IVT RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 80% correctly capped and 20% uncapped.
C-MMA6071010 reactions$323


AmpliCap-Max™ T7 High Yield Message Maker Kit  (PDF)

For co-transcriptional capping of IVT RNA with T7 RNA polymerase and standard cap analog. A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped.
C-ACM0403725 reactions$368


AmpliCap™ SP6 High Yield Message Maker Kit  (PDF)

For co-transcriptional capping of IVT RNA with SP6 RNA polymerase and standard cap analog. A reaction produces 35 µg RNA from 1 µg DNA in 2 hours. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped.
C-AC070625 reactions$368


Post-Transcriptional Tailing


A-Plus™ Poly(A) Polymerase Tailing Kit  (PDF)

For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAs
in vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods.
C-PAP5104H50 reactions$219


Modified NTP In Vitro Transcription


INCOGNITO™ T7 Ψ-RNA Transcription Kit  (PDF)

For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.
C-ICTY11051010 reactions$410


INCOGNITO™ SP6 Ψ-RNA Transcription Kit  (PDF)

For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1 µg DNA in 2 hours.
C-ICSY11051010 reactions$410


INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit  (PDF)

For the production of 5-methylcytosine- pseudouridine-containing, in vitrotranscribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours.
C-ICTMY11051010 reactions$691


INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit  (PDF)

For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing,
in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40 µg RNA from 1 µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped.
C-ICTAMY11051010 reactions$842


mRNA Decapping


Cap-Clip™ Acid Pyrophosphatase  (PDF)

For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.
C-CC15011H100 units$550


RNase Inhibition


ScriptGuard™ RNase Inhibitor  (PDF)

Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.
C-SRI6310K10,000 units$278

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